DNA

Part:BBa_J119011:Design

Designed by: Zachary Caton   Group: Eckdahl Lab   (2011-08-03)


BsaI-GFP2 (BsaI removed)-BsmBI


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 452
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 452
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 452
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1
    Illegal PstI site found at 452
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 7


Design Notes

This is NOT a BioBrick compatible part. It was designed to solve Hamiltonian Path Problems using multiple genes.

There is a rogue BsaI site at position 304 causing 457 bp to split into 304 bp and 153 bp. It can be removed or altered to lessen the occurrence of additional cutting at that site.



Source

PCR Amplification of existing part: GFP (E0040)split 351 bp from the beginning of the gene at with the sticky word ACCC

References